Wholemount in situ Hybridisation With Two Riboprobes

Double Labelled Limb Transverse Section Frontal Section Sagittal Section

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Image shows reconstructed virtual sections in transverse, frontal and sagittal planes from a double fluorescent wholemount in situ hybridisation on E12.5 mouse embryo with two riboprobes. Fluorescein labelled Sox-9 visualised with Alexafluor 488 (Invitrogen) shown in green, and DIG labelled Msx-1 visualised with Alexafluor 568 (Invitrogen) shown in red.
Courtesy of MRCT.

3D movie of this specimen can be seen here.

 

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