Wholemount in situ Hybridisation Followed by Immunohistochemistry
Click preferred orientation to view movie of virtual sections (3.8MB, 2.5MB, 2.1MB).
Image shows reconstructed virtual sections in transverse, frontal and sagittal planes from a wholemount in situ hybridisation on a E10.5 mouse embryo with Sox-9 DIG labelled riboprobe visualised with Alexafluor 568 (Invitrogen) shown in red, followed by fluorescent immunohistochemistry using monoclonal anti-neurofilament 160 (Sigma) visualised with Alexafluor 488 (Invitrogen) shown in green.
Courtesy of MRCT.
3D movie of this embryo can be seen here.