Wholemount in situ Hybridisation Followed by Immunohistochemistry

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

 

3D movie from a wholemount in situ hybridisation on a E10.5 mouse embryo with Sox-9 DIG labelled riboprobe visualised with Alexafluor 568 (Invitrogen) shown in red, followed by fluorescent immunohistochemistry using monoclonal anti-neurofilament 160 (Sigma) visualised with Alexafluor 488 (Invitrogen) shown in green.
Courtesy of MRCT.

This is a bespoke movie and not part of the Standard Imaging Package.

Movies of virtual sections of this specimen can be seen here.

See specimen before OPT

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