Wholemount and Immunofluorescence
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Image shows reconstructed virtual sections in transverse, frontal and sagittal planes from a wholemount in situ hybridisation on a E10.5 mouse embryo with Sox-9 DIG labelled riboprobe visualised with Alexafluor 568 (Invitrogen), followed by Pax-6 antibody visualised with Alexafluor 488 (Invitrogen).
Courtesy of MRCT.