Examples of OPT Imaging Package
A standard OPT imaging package consists of a number of movies (3D movies, virtual section movies, and original projection images) of merged and unmerged (channel) data, plus png stacks of image files for selected movies (see table).
Movies shown are created with our in-house software that is only available through our scanning service.
Contact us for details of our bespoke service.
We have used both surface and volume rendering styles to produce our standard movies, and the OPT Imaging Package includes examples of each (see below).
Volume
Surface
Anatomy
Merged Colour Virtual Section Movies
Channel Movies (unmerged monochrome virtual section movies and original projection image).
Example 1 shows wholemount in situ hybridisation on a E10.5 mouse embryo with Sox-9 DIG labelled riboprobe visualised with Alexafluor 568 (Invitrogen) shown in red, followed by fluorescent immunohistochemistry using anitbodies to Pax-6 visualised with Alexafluor 488 (Invitrogen) shown in green.
Example 2 shows wholemount in situ hybridisation on a E12.5 mouse embryo with Sox-9 DIG labelled riboprobe, visualised with Alexafluor-568 (Invitrogen), shown in red.
Example 3 shows fluorescent immunohistochemistry on a E12.5 mouse embryo using monoclonal anti-neurofilament 160 (Sigma) visualised with Alexafluor 488 (Invitrogen) shown in green.
Example 4 shows fluorescent immunohistochemistry on a E12.5 mouse embryo using rat monoclonal anti-CD31 (Ab-cam) visualised with Alexafluor 568 (Invitrogen) shown in red.
Click on the thumbnail to see movies. This page will be updated soon to show other types of sample, and more movies.
At the moment Example 3, and channel movies have examples for all movie styles.
Movie Styles Included in Package |
Example 1 |
Example 2 |
Example 3 |
Example 4 |
High resolution with signal volume rendered, png image files included. |
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High resolution, signal volume rendered, close up of top of sample. |
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High resolution, signal volume rendered, close up of middle of sample. |
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High resolution, signal volume rendered, close up of bottom of sample. |
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Signal only, volume rendered, png image files included. |
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Example 1 |
Example 2 |
Example 3 |
Example 4 |
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High resolution with signal surface rendered, png image files included. |
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High resolution, signal surface rendered, close up of top of sample. |
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High resolution, signal surface rendered, close up of middle of sample. |
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High resolution, signal surface rendered, close up of bottom of sample. |
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Signal only, surface rendered, png image files included. |
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Example 1 |
Example 2 |
Example 3 |
Example 4 |
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Anatomy only, with cut aways, png image files included. |
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Anatomy only, with 360° rotation, png image files included. |
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In addition to the above movies each OPT Imaging Package contains the following unmerged virtual section movies and Original Projection Images for each channel scanned e.g. GFP1, TXR etc as shown below.
Monochrome Unmerged Virtual Section Movies and Original Projection Images for each Channel |
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Monochrome Virtual Section Movie in Transverse Orientation |
Monochrome Virtual Section Movie in Sagittal Orientation |
Monochrome Virtual Section Movie in Frontal Orientation |
Original Projection Image |
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