Viewing Results: 1

Key:
a - Merged colour virtual section in sagittal plane
b - Monochrome virtual section in sagittal plane showing texas red (red) channel.
c - Monochrome virtual section in sagittal plane showing GFP2 (green) channel .
d - Monochrome virtual section in sagittal plane showing GFP1 channel indicating the anatomy, seen as blue in merged virtual sections.
e - Frame from 3D movie of merged data.

In order to produce our results we scan each specimen in different channels, i.e. with a specific filter to pick up a particular wavelength. For example a flourescently stained sample (as illustrated) is scanned with GFP1 channel (to show anatomy) ; Texas red (to show red signal); GFP2 channel (to show green signal).

Virtual sections for each channel are illustrated. These are formatted into movies for ease of viewing. Frames in each movie are numbered (at bottom left corner) and correspond to high quality png image files (see fig 3b here).  Scans from each channel are then merged to form colour virtual sections, as illustrated in fig 1a above. Frames in the colour virtual section movies are also numbered and correspond to high quality png files.

Fig e shows a frame from a 3D  movie, created from the merged data.
We use a variety of rendering styles and cut aways to produce our movies. The anatomy in this particular movie has been surfaced rendered and cut away, the green signal is also surface rendered, whilst the red signal has been volume rendered (compare this to fig 4a here, where both signals are volume rendered).